Hmdb loader

Showing Protein Nuclear inhibitor of protein phosphatase 1 (HMDBP08791)

IdentificationBiological propertiesGene propertiesProtein propertiesExternal linksReferencesXMLShow 2 metabolites
Identification
HMDB Protein ID HMDBP08791
Secondary Accession Numbers
  • 14514
Name Nuclear inhibitor of protein phosphatase 1
Synonyms
  1. ARD-1
  2. Activator of RNA decay
  3. NIPP-1
  4. Protein phosphatase 1 regulatory inhibitor subunit 8
Gene Name PPP1R8
Protein Type Unknown
Biological Properties
General Function Involved in DNA binding
Specific Function Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing
Pathways Not Available
Reactions Not Available
GO Classification Not Available
Cellular Location
  1. Isoform Gamma:Cytoplasm
Gene Properties
Chromosome Location Chromosome:1
Locus 1p35
SNPs PPP1R8
Gene Sequence 
>1056 bp
ATGGCGGCAGCCGCGAACTCCGGCTCTAGCCTCCCGCTGTTCGACTGCCCAACCTGGGCA
GGTAAGCCCCCTCCCGGTTTACATCTGGATGTAGTCAAAGGAGACAAACTAATTGAGAAA
CTGATTATTGATGAGAAGAAGTATTACTTATTTGGGAGAAACCCTGATTTGTGTGACTTT
ACCATTGACCACCAGTCTTGCTCTCGGGTCCATGCTGCACTTGTCTACCACAAGCATCTG
AAGAGAGTTTTCCTGATAGATCTCAACAGTACACACGGCACTTTCTTGGGTCACATTCGG
TTGGAACCTCACAAGCCTCAGCAAATTCCCATCGATTCCACGGTCTCATTTGGCGCATCC
ACAAGGGCATACACTCTGCGCGAGAAGCCTCAGACATTGCCATCGGCTGTGAAAGGAGAT
GAGAAGATGGGTGGAGAGGATGATGAACTCAAGGGCTTACTGGGGCTTCCAGAGGAGGAA
ACTGAGCTTGATAACCTGACAGAGTTCAACACTGCCCACAACAAGCGGATTTCTACCCTT
ACCATTGAGGAGGGAAATCTGGACATTCAAAGACCAAAGAGGAAGAGGAAGAACTCACGG
GTGACATTCAGTGAGGATGATGAGATCATCAACCCAGAGGATGTGGATCCCTCAGTTGGT
CGATTCAGGAACATGGTGCAAACTGCAGTGGTCCCAGTCAAGAAGAAGCGTGTGGAGGGC
CCTGGCTCCCTGGGCCTGGAGGAATCAGGGAGCAGGCGCATGCAGAACTTTGCCTTCAGC
GGAGGACTCTACGGGGGCCTGCCCCCCACACACAGTGAAGCAGGCTCCCAGCCACATGGC
ATCCATGGGACAGCACTCATCGGTGGCTTGCCCATGCCATACCCAAACCTTGCCCCTGAT
GTGGACTTGACTCCTGTTGTGCCGTCAGCAGTGAACATGAACCCTGCACCAAACCCTGCA
GTCTATAACCCTGAAGCTGTAAATGAACCCAAGAAGAAGAAATATGCAAAAGAGGCTTGG
CCAGGCAAGAAGCCCACACCTTCCTTGCTGATTTGA
Protein Properties
Number of Residues 351
Molecular Weight 38478.5
Theoretical pI 7.42
Pfam Domain Function
Signals
  • None
Transmembrane Regions
  • None
Protein Sequence 
>Nuclear inhibitor of protein phosphatase 1
MAAAANSGSSLPLFDCPTWAGKPPPGLHLDVVKGDKLIEKLIIDEKKYYLFGRNPDLCDF
TIDHQSCSRVHAALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQIPIDSTVSFGAS
TRAYTLREKPQTLPSAVKGDEKMGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTL
TIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRVEG
PGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPD
VDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLI
GenBank ID Protein 4883485
UniProtKB/Swiss-Prot ID Q12972
UniProtKB/Swiss-Prot Entry Name PP1R8_HUMAN
PDB IDs Not Available
GenBank Gene ID AF061958
GeneCard ID PPP1R8
GenAtlas ID PPP1R8
HGNC ID HGNC:9296
References
General References
  1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [PubMed:15489334 ]
  2. Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, Scott CE, Howe KL, Woodfine K, Spencer CC, Jones MC, Gillson C, Searle S, Zhou Y, Kokocinski F, McDonald L, Evans R, Phillips K, Atkinson A, Cooper R, Jones C, Hall RE, Andrews TD, Lloyd C, Ainscough R, Almeida JP, Ambrose KD, Anderson F, Andrew RW, Ashwell RI, Aubin K, Babbage AK, Bagguley CL, Bailey J, Beasley H, Bethel G, Bird CP, Bray-Allen S, Brown JY, Brown AJ, Buckley D, Burton J, Bye J, Carder C, Chapman JC, Clark SY, Clarke G, Clee C, Cobley V, Collier RE, Corby N, Coville GJ, Davies J, Deadman R, Dunn M, Earthrowl M, Ellington AG, Errington H, Frankish A, Frankland J, French L, Garner P, Garnett J, Gay L, Ghori MR, Gibson R, Gilby LM, Gillett W, Glithero RJ, Grafham DV, Griffiths C, Griffiths-Jones S, Grocock R, Hammond S, Harrison ES, Hart E, Haugen E, Heath PD, Holmes S, Holt K, Howden PJ, Hunt AR, Hunt SE, Hunter G, Isherwood J, James R, Johnson C, Johnson D, Joy A, Kay M, Kershaw JK, Kibukawa M, Kimberley AM, King A, Knights AJ, Lad H, Laird G, Lawlor S, Leongamornlert DA, Lloyd DM, Loveland J, Lovell J, Lush MJ, Lyne R, Martin S, Mashreghi-Mohammadi M, Matthews L, Matthews NS, McLaren S, Milne S, Mistry S, Moore MJ, Nickerson T, O'Dell CN, Oliver K, Palmeiri A, Palmer SA, Parker A, Patel D, Pearce AV, Peck AI, Pelan S, Phelps K, Phillimore BJ, Plumb R, Rajan J, Raymond C, Rouse G, Saenphimmachak C, Sehra HK, Sheridan E, Shownkeen R, Sims S, Skuce CD, Smith M, Steward C, Subramanian S, Sycamore N, Tracey A, Tromans A, Van Helmond Z, Wall M, Wallis JM, White S, Whitehead SL, Wilkinson JE, Willey DL, Williams H, Wilming L, Wray PW, Wu Z, Coulson A, Vaudin M, Sulston JE, Durbin R, Hubbard T, Wooster R, Dunham I, Carter NP, McVean G, Ross MT, Harrow J, Olson MV, Beck S, Rogers J, Bentley DR, Banerjee R, Bryant SP, Burford DC, Burrill WD, Clegg SM, Dhami P, Dovey O, Faulkner LM, Gribble SM, Langford CF, Pandian RD, Porter KM, Prigmore E: The DNA sequence and biological annotation of human chromosome 1. Nature. 2006 May 18;441(7091):315-21. [PubMed:16710414 ]
  3. Vulsteke V, Beullens M, Boudrez A, Keppens S, Van Eynde A, Rider MH, Stalmans W, Bollen M: Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1. J Biol Chem. 2004 Mar 5;279(10):8642-7. Epub 2003 Dec 29. [PubMed:14699119 ]
  4. Trinkle-Mulcahy L, Sleeman JE, Lamond AI: Dynamic targeting of protein phosphatase 1 within the nuclei of living mammalian cells. J Cell Sci. 2001 Dec;114(Pt 23):4219-28. [PubMed:11739654 ]
  5. Wang M, Cohen SN: ard-1: a human gene that reverses the effects of temperature-sensitive and deletion mutations in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10591-5. [PubMed:7524097 ]
  6. Van Eynde A, Perez-Callejon E, Schoenmakers E, Jacquemin M, Stalmans W, Bollen M: Organization and alternate splice products of the gene encoding nuclear inhibitor of protein phosphatase-1 (NIPP-1). Eur J Biochem. 1999 Apr;261(1):291-300. [PubMed:10103062 ]
  7. Van Eynde A, Wera S, Beullens M, Torrekens S, Van Leuven F, Stalmans W, Bollen M: Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing. J Biol Chem. 1995 Nov 24;270(47):28068-74. [PubMed:7499293 ]
  8. Claverie-Martin F, Wang M, Cohen SN: ARD-1 cDNA from human cells encodes a site-specific single-strand endoribonuclease that functionally resembles Escherichia coli RNase E. J Biol Chem. 1997 May 23;272(21):13823-8. [PubMed:9153239 ]
  9. Jin Q, Beullens M, Jagiello I, Van Eynde A, Vulsteke V, Stalmans W, Bollen M: Mapping of the RNA-binding and endoribonuclease domains of NIPP1, a nuclear targeting subunit of protein phosphatase 1. Biochem J. 1999 Aug 15;342 ( Pt 1):13-9. [PubMed:10432294 ]
  10. Chang AC, Sohlberg B, Trinkle-Mulcahy L, Claverie-Martin F, Cohen P, Cohen SN: Alternative splicing regulates the production of ARD-1 endoribonuclease and NIPP-1, an inhibitor of protein phosphatase-1, as isoforms encoded by the same gene. Gene. 1999 Nov 15;240(1):45-55. [PubMed:10564811 ]
  11. Beullens M, Vulsteke V, Van Eynde A, Jagiello I, Stalmans W, Bollen M: The C-terminus of NIPP1 (nuclear inhibitor of protein phosphatase-1) contains a novel binding site for protein phosphatase-1 that is controlled by tyrosine phosphorylation and RNA binding. Biochem J. 2000 Dec 15;352 Pt 3:651-8. [PubMed:11104670 ]
  12. Boudrez A, Beullens M, Groenen P, Van Eynde A, Vulsteke V, Jagiello I, Murray M, Krainer AR, Stalmans W, Bollen M: NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry. J Biol Chem. 2000 Aug 18;275(33):25411-7. [PubMed:10827081 ]
  13. Jagiello I, Van Eynde A, Vulsteke V, Beullens M, Boudrez A, Keppens S, Stalmans W, Bollen M: Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1. J Cell Sci. 2000 Nov;113 Pt 21:3761-8. [PubMed:11034904 ]
  14. Beullens M, Bollen M: The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly. J Biol Chem. 2002 May 31;277(22):19855-60. Epub 2002 Mar 21. [PubMed:11909864 ]
  15. Boudrez A, Beullens M, Waelkens E, Stalmans W, Bollen M: Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1. J Biol Chem. 2002 Aug 30;277(35):31834-41. Epub 2002 Jun 24. [PubMed:12105215 ]
  16. Jin Q, van Eynde A, Beullens M, Roy N, Thiel G, Stalmans W, Bollen M: The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor. J Biol Chem. 2003 Aug 15;278(33):30677-85. Epub 2003 Jun 3. [PubMed:12788942 ]